Recent Publications

Cheung, S. T.; Miller, M. S.; Pacoma, R.; Roland, J.; Liu, J.; Schumacher, A. M.; Hsieh-ˇWilson, L. C. “Discovery of a Small-ˇMolecule Modulator of Glycosaminoglycan Sulfation,” ACS Chem Biol. 2017, 12, 3126-3133.

Wang AC, Jensen EH, Rexach JE, Vinters HV, Hsieh-Wilson LC. "Loss of O-GlcNAc glycosylation in forebrain excitatory neurons induces neurodegeneration." Proceedings of the National Academy of Sciences. 2016 Dec 27;113(52):15120-5.

Griffin ME, Hsieh-Wilson, LC. “Glycan engineering for cell and developmental biology.” Cell Chem Biol. 2016, 23(1):108-121.

Griffin ME, Jensen EH, Mason DE, Jenkins CL, Stone SE, Peters EC, Hsieh-Wilson LC. “Comprehensive mapping of O-GlcNAc modification sites using a chemically cleavable tag.” Mol Biosyst. 2016, 12(6): 1756-1759.

Miller, G. M.; Hsieh-Wilson, L. C.; "Sugar-dependent modulation of neuronal development, regeneration, and plasticity by chondroitin sulfate proteoglycans," Experimental Neurology. 2015.

Pulsipher A.; Griffin M. E.;  Stone S. E.; Hsieh-Wilson L. C.; "Long-lived engineering of glycans to direct stem cell fate." Angew Chem Int Ed Engl. 2015, 54, 1466-1470.

Matho M. H.; de Val N.; Miller G. M.; Brown J.; Schlossman A.; Meng X.; Crotty S.; Peters B.; Xiang Y.; Hsieh-Wilson L. C.; Ward A. B.; Zajonc D. M.; "Murine anti-vaccinia virus D8 antibodies target different epitopes and differ in their ability to block D8 binding to CS-E," PLoS Pathog. 2014, 10, e1004495.

Wibowo A.; Peters E. C.; Hsieh-Wilson L. C.; "Photoactivatable glycopolymers for the proteome-wide identification of fucose-α(1-2)-galactose binding proteins," J. Am. Chem. Soc. 2014, 136, 9528-9531.

Pulsipher, A.; Griffin, M. E.; Stone, S. E.; Brown, J. M.; Hsieh-Wilson, L. C. "Directing Neuronal Signaling through Cell-Surface Glycan Engineering," J. Am. Chem. Soc. 2014, 136, 6794-6797.

Hsieh-Wilson, L. C.; Griffin, M. E. "Improving Biologic Drugs via Total Chemical Synthesis," Science 2013, 342, 1332-1333.

Clark, P. M.; Rexach, J. E.; Hsieh-Wilson, L. C. "Visualization of O-GlcNAc Glycosylation Stoichiometry and Dynamics using Resolvable Poly(ethylene glycol) Mass Tags,"  Curr. Protocols Chem. Biol. 2013, 5, 281-302.

Griffin, M. E.; Hsieh-Wilson, L. C. "Synthetic Probes of Glycosaminoglycan Function," Curr. Op. Chem. Biol. 2013, 17, 1014-1022.

Oh, Y. I.; Sheng, G.; Chang, S.-K.; Hsieh-Wilson, L. C. "Tailored Glycopolymers as Anticoagulant Heparin Mimetics," Angew. Chem. Int. Ed.  2013, 52, 11796-11799.

Sheng, G.; Oh, Y. I.; Chang, S.-K.; Hsieh-Wilson, L. C. "Tunable Heparan Sulfate Mimetics for Modulating Chemokine Activity," J. Am. Chem. Soc. 2013, 135, 10898-10901.

Dick, G.; Tan, C. l.; Alves, J. N.; Ehlert, E.; Miller, G.; Hsieh-Wilson, L. C.; Sugahara, K.; Oosterhof, A.; van Kuppevelt, T. H.; Verhaagen, J.; Fawcett, J. W.; Kwok, J. C. F. "Semaphorin 3A Binds to the Perineuronal Nets via Chondroitin Sulfate Type E (CS-E) Motifs in Rodent Brains," J. Biol. Chem. 2013, 288, 27384-27395.

Ramakrishnan, P.; Clark, P. M.; Mason, D. E.; Peters, E. C.; Hsieh-Wilson, L. C.; Baltimore, D. "Activation of the Transcriptional Function of the NF-kB Protein c-Rel by O-GlcNAc Glycosylation," Science Signaling2013, 6 ra75.

Yi, W; Clark, P. M.; Mason, D. E.; Keenan, M. C.; Hill. C.; Goddard, W. A., III; Peters, E. C.; Driggers, E. M.; Hsieh-Wilson, L. C. "Phosphofructokinase 1 Glycosylation Regulates Cell Growth and Metabolism," Science 2012, 337, 975–980.
Featured in:
Mattaini, K. R.; Vander Heiden, M. G. "Glycosylation to Adapt to Stress," Science 2012, 337, 925–926.
Borman, S. "Glycosylation Linked to Cancer," Chem. Eng. News 2012, 90, 9.
Goodman, C. "A GlcNAc Switch," Nat. Chem. Biol. 2012, 8, 809.
Ray, L. B. "Metabolic Sensor," Sci. Signal. 2012, 5, ec225.

Chaubard, J.-L.; Krishnamurthy, C.; Yi, W.; Smith, D.; Hsieh-Wilson, L. C. "Chemoenzymatic Probes for Detecting and Imaging Fucose-α(1-2)-Galactose Glycan Biomarkers," J. Am. Chem. Soc. 2012, 134, 4489–4492.

Brown, J. M.; Xia, J.; BinQuan, Z.; Cho, K.-S.; Rogers, C. J.; Gama, C. I.; Rawat, M.; Tully, S. E.; Uetani, N.; Mason, D.; Tremblay, M. L.; Peters, E. C.; Habuchi, O.; Chen, D. F.; Hsieh-Wilson, L. C. "A Sulfated Carbohydrate Epitope Inhibits Axon Regeneration After Injury," Proc. Natl. Acad. Sci. U. S. A. 2012, 109, 4768–4773.

Rexach, J. E.; Clark, P. M.; Mason, D. E.; Neve, R. L.; Peters, E. C.; Hsieh-Wilson, L. C. "Dynamic O-GlcNAc modification regulates CREB-mediated gene expression and memory formation," Nature Chem. Biol. 2012, 8, 253–261.

Rogers, C. J.; Hsieh-Wilson, L. C. "Microarray method for the rapid detection of glycosaminoglycan-protein interactions," Carbohydrate Microarrays: Methods and Protocols, Methods in Molecular Biology 2012, 808, 321–336.

Rogers, C. J.; Clark, P. M.; Tully, S. E.; Abrol, R.; Garcia, K. C.; Goddard, W. A., III; Hsieh-Wilson, L. C. "Elucidating glycosaminoglycan-protein-protein interactions using carbohydrate microarray and computational approaches," Proc. Natl. Acad. Sci. U. S. A. 2011, 108, 9747–9752.

Lee, S.-G.; Brown, J. M.; Rogers, C. J.; Matson, J. B.; Krishnamurthy, C.; Rawat, Manish; Hsieh-Wilson, L. C. "End-functionalized glycopolymers as mimetics of chondroitin sulfate proteoglycans," Chem. Sci. 2010, 1, 322–325.

Rexach, J. E.; Rogers, C. J.; Yu, S.-H.; Tao, J.; Sun, Y.; Hsieh-Wilson, L. C. "Quantification of O-Glycosylation Stoichiometry and Dynamics using Resolvable Mass Tags," Nature Chem. Biol. 2010, 6, 645–651. Featured in Nature Chem. Biol. 2010, 6, 634–635.

Murrey, H. E.; Ficarro, S. B.; Krishnamurthy, C.; Domino, S. E.; Peters, E. C.; Hsieh-Wilson, L. C. "Identification of the Plasticity-Relevant Fucose-α(1-2)-Galactose Proteome from Mouse Olfactory Bulb," Biochemistry 2009, 48, 7261–7270.

Wang, Z.; Park, K.; Comer F.; Hsieh-Wilson, L. C.; Saudek, C. D., Hart, G. W. "Site-Specific GlcNAcylation of Human Erythrocyte Proteins: Potential Biomarker(s) for Diabetes Mellitus," Diabetes 2009, 58, 309–317.

Clark, P. M.; Dweck, J. F.; Mason, D.; Hart, C.; Buck, S. B.; Peters, E. C.; Agnew, B. J.; Hsieh-Wilson, L. C. "Direct In-Gel Fluorescence Detection and Cellular Imaging of O-GlcNAc-Modified Proteins," J. Am. Chem. Soc. 2008, 130, 11576–11577.

Murrey, H. E.; Hsieh-Wilson, L. C. "The Chemical Neurobiology of Carbohydrates," Chem. Rev. 2008, 108, 1708–1731.

Rexach, J. E.; Clark, P. M.; Hsieh-Wilson, L. C. "Chemical Approaches to Understanding O-GlcNAc Glycosylation in the Brain," Nature Chem. Biol. 2008, 4, 97–106.

Rawat, M.; Gama, C . I.; Matson, J. B.; Hsieh-Wilson, L. C. "Neuroactive Chondroitin Sulfate Glycomimetics," J. Am. Chem. Soc. 2008, 130, 2959–2961. Featured in Chem. Eng. News. 2009, 87, 28.

Sotogaku, N.; Tully, S. E.; Gama, C. I.; Higashi, H.; Tanaka, M.; Hsieh-Wilson, L. C.; Nishi, A., "Activation of Phospholipase C Pathways by Synthetic Chondroitin Sulfate-E Tetrasaccharide Promotes the Neurite Outgrowth of Dopaminergic Neurons," J. Neurochem. 2007, 103, 749–760.

Khidekel, N.; Ficarro, S. B.; Clark, P. M.; Bryan, M.; Swaney, D. L.; Rexach, J.; Sun, Y. E.; Coon, J. J.; Peters, E. C; Hsieh-Wilson, L. C. "Probing the Dynamics of O-GlcNAc Glycosylation in the Brain using Quantitative Proteomics," Nature Chem. Biol. 2007, 3, 339–348. Featured in Nature Chem. Biol. 2007, 3, 303–304 and Nature 2007, 447, 510–511.

Boeggeman, E.; Ramakrishnan, B.; Kilgore, C.; Khidekel, N.; Hsieh-Wilson, L. C.; Simpson, J. T.; Qasba P. K. "Direct Identification of Nonreducing GlcNAc Residues on N-Glycans of Glycoproteins Using a Novel Chemoenzymatic Method," Bioconjug. Chem. 2007, 18, 806–814.

Shipp, E. L.; Hsieh-Wilson, L. C. "Profiling the Sulfation Specificities of Glycosaminoglycan Interactions with Growth Factors and Chemotactic Proteins Using Microarrays," Chem. Biol. 2007, 14, 195–208. Featured in Chem. Biol. 2007, 14, 119–120.

Hsieh-Wilson, L. C. "A Sweet Synthesis," Nature 2007, 445, 31–33.

Gama, C. I.; Tully, S. E.; Sotogaku, N.; Clark, P. M.; Rawat, M.; Vaidehi, N.; Goddard, W. A., III; Nishi, A.; Hsieh-Wilson, L. C. "Sulfation Patterns of Glycosaminoglycans Encode Molecular Recognition and Activity," Nature Chem. Biol. 2006, 2, 467–473. Featured in Nature Chem. Biol. 2006, 2, 449–450, Nature Methods 2006, 3, 769, and Chem. Eng. News 2006, 84, 48.

Tully, S. E.; Rawat, M.; Hsieh-Wilson, L. C. "Discovery of a TNF-α Antagonist Using Chondroitin Sulfate Microarrays," J. Am. Chem. Soc. 2006, 128, 7740–7741.

Murrey, H.; Gama, C. I.; Kalovidouris, S. A.; Luo, W.-I.; Hsieh-Wilson, L. C. "Protein Fucosylation Regulates Synapsin Ia/Ib Expression and Neuronal Morphology in Primary Hippocampal Neurons," Proc. Natl. Acad. Sci. U.S.A. 2006, 103, 21–26. Featured in "This Week in PNAS," Sci. STKE 2006, 472, 317 and Nature Chem. Biol. 2006, 2, 67.

Gama, C. I.; Hsieh-Wilson, L. C. "Chemical approaches to deciphering the glycosaminoglycan code," Curr. Op. Chem. Biol. 2005, 9, 609–619.

Kalovidouris, S.; Gama, C. I.; Hsieh-Wilson, L. C. "A Role for Fucose α(1-2) Galactose Carbohydrates in Neuronal Growth," J. Am. Chem. Soc. 2005, 127, 1340–1341.

Khidekel, N.; Ficarro, S. B.; Peters, E. C; Hsieh-Wilson, L. C. "Exploring the O-GlcNAc Proteome: Direct Identification of O-GlcNAc-Modified Proteins," Proc. Natl. Acad. Sci. U.S.A. 2004, 101, 13132–13137. Featured in Nature Methods 2004, 1, 5.

Hsieh-Wilson, L. C. "Tailor-made Glycoproteins," Trends Biotechnol. 2004, 22, 489–491.

Tai, H.-C.; Khidekel, N.; Ficarro, S. B.; Peters, E. C; Hsieh-Wilson, L. C. "Parallel Identification of O-GlcNAc-Modified Proteins from Cell Lysates," J. Am. Chem. Soc. 2004, 126, 10500–10501.

Tully, S. E.; Mabon, R.; Gama, C. I.; Tsai, S. L.; Liu, X.; Hsieh-Wilson, L. C. "A Chondroitin Sulfate Small Molecule that Stimulates Neuronal Outgrowth," J. Am. Chem. Soc. 2004, 126, 7736–7737. Featured in Chem. Eng. News. 2004, 82, 9.

Khidekel, N.; Hsieh-Wilson, L. C. "A 'Molecular Switchboard' – Covalent Modifications to Proteins and Their Impact on Transcription," Org. Biomol. Chem. 2004, 2, 1–7.

Khidekel, N.; Arndt, S.; Lamarre-Vincent, N.; Lippert, A.; Poulin-Kerstien, K. G.; Ramakrishnan, B.; Qasba, P. K.; Hsieh-Wilson, L. C. "A Chemoenzymatic Approach Toward the Rapid and Sensitive Detection of O-GlcNAc Posttranslational Modifications," J. Am. Chem. Soc. 2003, 125, 16162–16163.

Arndt, S. E.; Hsieh-Wilson, L. C. "Use of Cerny Expoxides for the Accelerated Synthesis of Glycosaminoglycans," Org. Lett. 2003, 5, 4179–4182.

Lamarre-Vincent, N.; Hsieh-Wilson, L. C. "Dynamic Glycosylation of the Transcription Factor CREB: A Potential Role in Gene Regulation," J. Am. Chem. Soc. 2003, 125, 6612–6613. Featured in Chem. Eng. News 2003, 81, 21.

Other selected publications

Uematsu, K; Futter, M; Hsieh-Wilson, L. C.; Higashi, H.; Maeda, H.; Narin, A. C.; Greengard, P.; Nishi, A. "Regulation of Spinophilin Ser94 Phosphorylation in Neostriatal Neurons Involves Both DARPP-32-Dependent and Independent Pathways," J. Neurochem. 2005, 95, 1642–1652.

Grossman, S. D.; Hsieh-Wilson, L. C.; Allen, P. B.; Nairn, A. C.; Greengard, P. "The Actin-Binding Domain of Spinophilin is Necessary and Sufficient for Targeting to Dendritic Spines," NeuroMolec. Medicine 2002, 2, 61–69.

Watanabe, T.; Huang, H.-b.; Horiuchi, A.; Hsieh-Wilson, L. C.; Allen, P. B.; Shenolikar, S.; Greengard, P.; Nairn, A. C. "Protein Phosphatase 1 Regulation by Inhibitors and Targeting Subunits," Proc. Natl. Acad. Sci. USA 2001, 98, 3080–3085.

Allen, P. B.; Hsieh-Wilson, L.; Yan, Z.; Feng, J.; Ouimet, C. C.; Greengard, P. "Control of Protein Phosphatase-1 in the Dendrite," Biochem. Soc. Trans. 1999, 27, 543–546.

Yan, Z.; Hsieh-Wilson, L.; Feng, J.; Tomizawa, K.; Allen, P. B.; Fienberg, A. A.; Nairn, A. C.; Greengard, P. "Protein Phosphatase 1 Modulation of Neostriatal AMPA Channels: Regulation by DARPP-32 and Spinophilin," Nature Neurosci. 1999, 2, 13–17.

Hsieh-Wilson, L. C.; Allen, P. B.; Watanabe, T.; Nairn, A. C.; Greengard, P. "Characterization of the Neuronal Targeting Protein Spinophilin and Its Interactions with Protein Phosphatase-1," Biochemistry 1999, 38, 4365–4373.

Ma, L.; Hsieh-Wilson, L. C.; Schultz, P. G. "Antibody Catalysis of Peptidyl-Prolyl cis-trans Isomerization in the Folding of RNase T1," Proc. Natl. Acad. Sci. USA 1998, 95, 7251–7256.

Stevens, R. C.; Hsieh-Wilson, L. C.; Santarsiero, B. D.; Wedemayer, G. J.; Spiller, B.; Wang, L. H.; Barnes, D.; Ulrich, H. D.; Patten, P. A.; Romesberg, F. E.; Schultz, P. G. "Structural Studies of Catalytic Antibodies," Israel J. Chem. 1996, 36, 121–132.

Hsieh-Wilson, L. C.; Schultz, P. G.; Stevens, R. C. "Insights into Antibody Catalysis: Structure of an Oxygenation Catalyst at 1.9-Å Resolution," Proc. Natl. Acad. Sci. USA 1996, 93, 5363–5367.

Hsieh-Wilson, L. C.; Xiang, X.-D.; Schultz, P. G. "Lessons from the Immune System: From Catalysis to Materials Science," Acc. Chem. Res. 1996, 29, 164–170.

Hsieh, L. C.; Stephans, J. C.; Schultz, P. G. "An Efficient Antibody-Catalyzed Oxygenation Reaction," J. Am. Chem. Soc. 1994, 116, 2167–2168.

Yu, J.; Hsieh, L. C.; Kochersperger, L.; Yonkovich, S.; Stephans, J. C.; Gallop, M. A.; Schultz, P. G. "Progress Toward an Antibody Glycosidase," Angew. Chem. Int. Ed. Eng. 1994, 33, 339–340.

Hsieh, L. C.; Yonkovich, S.; Kochersperger, L.; Schultz, P. G. "Controlling Chemical Reactivity with Antibodies," Science 1993, 260, 337–340.