Supported phospholipid bilayers are formed by the self-assembly of lipids into two opposing leaflets on a hydrophilic surface. We have developed a novel lithographic technique to pattern artificial lipid microdomains by exploiting the limited mobility of gel-phase phospholipids in two-component mixtures [ChemPhysChem, in press]. The technique consists of creating either positive or negative pattern transfers in a gel phase phospholipid bilayer resist followed by backfilling of void spaces in the resist with vesicles containing liquid crystalline phase fluid lipids. The resulting patterns are mechanically stable for several days. We can specifically functionalize selected bilayer areas with fluorescently labeled streptavidin probes with minimal nonspecific binding. Moreover, we show that the patterned areas as well as the lateral barriers separating them form a cohesive and continuous supported lipid bilayer, which we will enable patterning these systems at the nanoscale, via nanografting or nanoshaving with an AFM tip.